polyclonal rabbit antibody human myd88  (Enzo Biochem)

Journal: Diabetology & Metabolic Syndrome

Article Title: High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy

doi: 10.1186/s13098-015-0086-4

Figure Lengend Snippet: Activation of MyD88 and cytokine expression in HMEC-1 cells under high glucose condition. a Western blotting was performed for MyD88 expression in the HMEC-1 cells after exposure to 15 and 25 mmol/l glucose for 12 h. β-actin was used as a control. b Cells were treated with 15 and 25 mmol/l glucose for 6 h. The mRNA for IL-1β was detected by quantitative RT-PCR and normalized to GAPDH. Asterisk indicates P < 0.05 compared to 5.5 mmol/l glucose. c The cells were stimulated with 15 mmol/l glucose for 0, 1, 3, 6, 12 and 24 h. The mRNA of IL-1β was detected by quantitative RT-PCR and normalized to GAPDH to the 0 h group and expressed as the mean ± SE. * P < 0.05 compared to the 0 h group. d IL-1β protein in the supernatants of HMEC-1 cells after high-glucose treatment for indicated time points measured using ELISA. Asterisk indicates P < 0.05 compared to 5.5 mmol/l glucose

Article Snippet: After being blocked with 5 % nonfat milk for 1 h, the membrane were incubated with the following primary antibodies at 4 °C overnight: polyclonal rabbit anti-human TLR4 (1:500; Boster Wuhan, China), polyclonal rabbit anti-human MyD88 (1:100; Boster Wuhan, China) and monoclonal mouse anti-β-actin (1:100; Boster, Wuhan, China).

Techniques: Activation Assay, Expressing, Western Blot, Control, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: Oncogene

Article Title: TLR4 signaling induced by lipopolysacharide or paclitaxel regulates tumor survival and chemoresistance in ovarian cancer

doi: 10.1038/onc.2009.289

Figure Lengend Snippet: Expression of TLR4 and MyD88 in ovarian cancer, benign tumors and normal controls a

Article Snippet: Tumor cells deposited on glass slides were fixed in 2% (w/v) paraformaldehyde in PBS for 15 min, permeablized with 0.1% (w/v) Triton X in PBS, washed and blocked with 2% (w/v) bovine serum albumin (BSA; Sigma) in PBS for 4 min. Polyclonal goat anti-human TLR4 antibodies (Abs) (Santa Cruz Biotechnology; 1:100) or polyclonal rabbit anti-human MyD88 Abs (Cell Signaling Technology; 1:100) served as primary reagents.

Techniques: Expressing

Journal: Oncogene

Article Title: TLR4 signaling induced by lipopolysacharide or paclitaxel regulates tumor survival and chemoresistance in ovarian cancer

doi: 10.1038/onc.2009.289

Figure Lengend Snippet: A ) Immunostaining for TLR4 in a normal ovarian tissue adjacent to tumor (1), benign tumor (2), borderline tumor (3) and ovarian cancer ( Adenocarcionoma serosum FIGO III) (4) (Mag. × 200, Inset × 600); Immunostaining for MyD88 in ovarian tissues adjacent to tumor (5), benign tumor (6), borderline tumor (7), ovarian cancer ( Adenocarcionoma serosum FIGO III) (8) (Mag. × 200, Inset × 600. B ) Immunofluorescence for TLR4 protein in an ovarian cancer cell line (SKOV3) (2) Negative staining for TLR4 using isotype control IgG (1). Immunofluorescence for MyD88 in ovarian carcinoma cell lines, SKOV3 (3) and A2780 (4) (× 400); C ) Expression of TLR4 mRNA and Western blot for TLR4 and MyD88 in ovarian cancer cell lines.

Article Snippet: Tumor cells deposited on glass slides were fixed in 2% (w/v) paraformaldehyde in PBS for 15 min, permeablized with 0.1% (w/v) Triton X in PBS, washed and blocked with 2% (w/v) bovine serum albumin (BSA; Sigma) in PBS for 4 min. Polyclonal goat anti-human TLR4 antibodies (Abs) (Santa Cruz Biotechnology; 1:100) or polyclonal rabbit anti-human MyD88 Abs (Cell Signaling Technology; 1:100) served as primary reagents.

Techniques: Immunostaining, Immunofluorescence, Negative Staining, Control, Expressing, Western Blot